Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
JM Zarling, M Shoyab, H Marquardt… - Proceedings of the …, 1986 - National Acad Sciences
JM Zarling, M Shoyab, H Marquardt, MB Hanson, MN Lioubin, GJ Todaro
Proceedings of the National Academy of Sciences, 1986•National Acad SciencesA polypeptide termed oncostatin M, which inhibits the replication of A375 melanoma and
other human tumor cells, but not normal human fibroblasts, has been isolated from serum-
free supernatants of U-937 histiocytic lymphoma cells that have been induced to differentiate
into macrophage-like cells following treatment with the phorbol ester phorbol 12-myristate
13-acetate. No such growth inhibitory activity is detected in the supernatant of untreated U-
937 cells, indicating that the protein is induced or increased in expression in the phorbol …
other human tumor cells, but not normal human fibroblasts, has been isolated from serum-
free supernatants of U-937 histiocytic lymphoma cells that have been induced to differentiate
into macrophage-like cells following treatment with the phorbol ester phorbol 12-myristate
13-acetate. No such growth inhibitory activity is detected in the supernatant of untreated U-
937 cells, indicating that the protein is induced or increased in expression in the phorbol …
A polypeptide termed oncostatin M, which inhibits the replication of A375 melanoma and other human tumor cells, but not normal human fibroblasts, has been isolated from serum-free supernatants of U-937 histiocytic lymphoma cells that have been induced to differentiate into macrophage-like cells following treatment with the phorbol ester phorbol 12-myristate 13-acetate. No such growth inhibitory activity is detected in the supernatant of untreated U-937 cells, indicating that the protein is induced or increased in expression in the phorbol ester-induced differentiated cells. Oncostatin M is stable between pH 2 and 11 and after heating for 1 hr at 56 degrees C but is not stable at 90 degrees C. Purification of oncostatin M has been achieved by gel chromatography and reversed-phase HPLC, using sequentially acetonitrile and n-propanol in the presence of aqueous trifluoroacetic acid. The apparent molecular weight of oncostatin M is approximately 18,000, as determined by gel chromatography, and 28,000, as determined by polyacrylamide gel electrophoresis. The amino-terminal amino acid sequence of the purified polypeptide has been determined. No substantial sequence homology between oncostatin M and other proteins was found, including other tumor cell inhibitory proteins produced by mononuclear cells. Oncostatin M, therefore, appears to represent a distinct cell growth regulator.
National Acad Sciences