[HTML][HTML] Mg2+ and Ca2+ differentially regulate DNA binding and dimerization of DREAM
DREAM (calsenilin/KChIP3) is an EF-hand calcium-binding protein that represses
transcription of prodynorphin and c-fos genes. Here we present structural and binding
studies on single-site mutants of DREAM designed to disable Ca 2+ binding to each of the
functional EF-hands (EF-2: D150N; EF-3: E186Q; and EF-4: E234Q). Isothermal titration
calorimetry (ITC) analysis of Ca 2+ binding to the various mutants revealed that, in the
absence of Mg 2+, Ca 2+ binds independently and sequentially to EF-3 (ΔH=-2.4 kcal/mol) …
transcription of prodynorphin and c-fos genes. Here we present structural and binding
studies on single-site mutants of DREAM designed to disable Ca 2+ binding to each of the
functional EF-hands (EF-2: D150N; EF-3: E186Q; and EF-4: E234Q). Isothermal titration
calorimetry (ITC) analysis of Ca 2+ binding to the various mutants revealed that, in the
absence of Mg 2+, Ca 2+ binds independently and sequentially to EF-3 (ΔH=-2.4 kcal/mol) …