[HTML][HTML] Simultaneous steady-state and dynamic 13C NMR can differentiate alternative routes of pyruvate metabolism in living cancer cells
C Yang, C Harrison, ES Jin, DT Chuang… - Journal of Biological …, 2014 - ASBMB
Metabolic reprogramming facilitates cancer cell growth, so quantitative metabolic flux
measurements could produce useful biomarkers. However, current methods to analyze flux
in vivo provide either a steady-state overview of relative activities (infusion of 13 C and
analysis of extracted metabolites) or a dynamic view of a few reactions (hyperpolarized 13 C
spectroscopy). Moreover, although hyperpolarization has successfully quantified pyruvate-
lactate exchanges, its ability to assess mitochondrial pyruvate metabolism is unproven in …
measurements could produce useful biomarkers. However, current methods to analyze flux
in vivo provide either a steady-state overview of relative activities (infusion of 13 C and
analysis of extracted metabolites) or a dynamic view of a few reactions (hyperpolarized 13 C
spectroscopy). Moreover, although hyperpolarization has successfully quantified pyruvate-
lactate exchanges, its ability to assess mitochondrial pyruvate metabolism is unproven in …
