Production of monoclonal antibodies
WM Yokoyama, M Christensen… - Current protocols in …, 2013 - Wiley Online Library
WM Yokoyama, M Christensen, GD Santos, D Miller, J Ho, T Wu, M Dziegelewski…
Current protocols in immunology, 2013•Wiley Online LibraryThis unit describes the production of monoclonal antibodies beginning with immunization,
cell fusion, and selection. Support protocols are provided for screening primary hybridoma
supernatants for antibodies of desired specificity, establishment of stable hybridoma lines,
cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation
of cloning/expansion medium. An alternate protocol describes cell fusion and one‐step
selection and cloning of hybridomas utilizing a semi‐solid methylcellulose‐based medium …
cell fusion, and selection. Support protocols are provided for screening primary hybridoma
supernatants for antibodies of desired specificity, establishment of stable hybridoma lines,
cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation
of cloning/expansion medium. An alternate protocol describes cell fusion and one‐step
selection and cloning of hybridomas utilizing a semi‐solid methylcellulose‐based medium …
Abstract
This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one‐step selection and cloning of hybridomas utilizing a semi‐solid methylcellulose‐based medium (ClonaCell‐HY from StemCell Technologies). Curr. Protoc. Immunol. 102:2.5.1‐2.5.29. © 2013 by John Wiley & Sons, Inc.
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